CD11b anticorps (PE)

N° du produit ABIN320197

Cet anticorps Rat Monoclonal détecte spécifiquement CD11b dans FACS. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour CD11b anticorps (PE) (ABIN320197)

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Clone: 5C6

Détail du produit anti-CD11b anticorps (PE)

Purification: Purified IgG prepared by ion exchange chromatography

Immunogène: Thioglycollate-elicted Peritoneal Macrophages (TPM). Spleen cells from AO rats were fused with cells of the Y3 rat myeloma cell line.

Isotype: IgG2b

Information d'application

Indications d'application: Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL distilled water.

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: CD11b

Sujet: CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8 % spleen cells, 44 % bone marrow cells and less than 1 % of thymocytes and is commonly used as a microglial marker in nervous tissue.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

ID gène: 10090

UniProt: P05555

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation